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protein band visualization  (Bio-Rad)


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    Structured Review

    Bio-Rad protein band visualization
    Protein Band Visualization, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 44933 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+band+visualization/Protein+Standard+I/pm40945645-89-18-26
    Average 99 stars, based on 44933 article reviews
    protein band visualization - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector
    Article Snippet: .. Western Blot Hyper HRP Substrate (Takara, Japan) was used for protein band visualization, and the blots were imaged with a Molecular Imager ® ChemiDocTM XRS + Imaging System (Bio-Rad Laboratories, CA, USA). ..

    Imaging:

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector
    Article Snippet: .. Western Blot Hyper HRP Substrate (Takara, Japan) was used for protein band visualization, and the blots were imaged with a Molecular Imager ® ChemiDocTM XRS + Imaging System (Bio-Rad Laboratories, CA, USA). ..

    Article Title: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy.
    Article Snippet: .. An ECL chemiluminescence substrate was later added for protein band visualization, whereas ChemiDoc XRS System (Bio-Rad, USA) was applied in imaging. .. Image Lab Software (Bio-Rad) was employed for quantifying band intensities, and GAPDH was an endogenous reference for normalization.

    Article Title: Hyperglycemia promotes tumor immune evasion via B7-H4 upregulation in ovarian cancer.
    Article Snippet: Background: The efficacy of ICB therapy has long been shown to be less prominent in patients with metabolic disorders, including type II diabetes, but the underlying mechanisms remain unclear.. Objective: To investigate how hyperglycemia influences tumor immune evasion and suppresses CD8 T cell function in ovarian cancer, focusing on the role of B7-H4 and AP-1.. Methods: The BKS db/db mice, a model of type II diabetes, and ID8 ovarian cancer cells were used to evaluate tumor growth, immune cell infiltration, and the impact of hyperglycemia on immune checkpoint expression.

    Article Title: The mechanism of action in Mussaenda pubescens (Yuye Jinhua) against influenza A virus: Evidence from in vitro and in vivo studies.
    Article Snippet: Background: Influenza A virus (IAV) is recognized as a predominant etiological agent responsible for severe contagious diseases in both humans and animals, characterized by elevated pathogenicity and mortality rates.. Mussaenda pubescens (Yuye Jinhua, YYJH), a traditional medicinal herb known for its anti-inflammatory and detoxifying properties, has been predominantly utilized in conjunction with other therapeutic modalities to manage influenza virus infections.. However, the standalone therapeutic efficacy and underlying mechanisms of YYJH against IAV infection remain insufficiently clarified.

    Article Title: Label-free nonlinear optical signatures of extracellular vesicles in liquid and tissue biopsies of human breast cancer
    Article Snippet: .. Resolved proteins were transferred onto 0.22 μm pore-size nitrocellulose membrane, which was subdivided into distinct molecular weight zones, blocked with 3% BSA solution for 2 h and individually immunoblotted with a 1:1000 dilution of either unconjugated Anti-ALIX rabbit monoclonal antibody (Boster-Bio, #M01751), anti-TSG101 rabbit polyclonal antibody (Abcam, #ab30871), anti-CD9 rabbit monoclonal antibody (Boster-Bio, #M01202), anti-CD81 rabbit monoclonal antibody (Boster-Bio, #M01281-1), Anti-GM130/GOLGA2 (Golgi organelle contamination indicator) mouse monoclonal 6D4 antibody (Boster-Bio, # M05865-2) or anti-Calreticulin/CALR (ER organelle presence indicator) (Boster-Bio, #A00894-1) antibody overnight followed by four 7 min wash cycles in prechilled 1X TBS-T buffer, probing with 1:10,000 horse anti-mouse IgG Cell Signaling Technology, #7076S) or 1:40,000 goat anti-rabbit IgG (H + L) (Thermo Fisher, #31460) HRP-linked secondary antibody for 1 h at room temperature, four 5 min washes in 1X TBS-T buffer and a final wash in dH2O prior to chemiluminescent signal development by a 5 min-long blot incubation with SuperSignalTM West Pico PLUS chemiluminescent substrate (Thermo Scientific) and automated protein band visualization in Bio-Rad ChemiDoc MP imaging system at optimal non-saturating 24 s (ALIX), 44 s (TSG101), 180 s (CD9, CD81, GM130 and CALR) exposure time. ..

    Article Title: Oroxin A alleviates early brain injury after subarachnoid hemorrhage by regulating ferroptosis and neuroinflammation.
    Article Snippet: .. The incubation occurred at ambient temperature for 1.5 h. The protein band visualization and quantification were carried out utilizing a Bio-Rad imaging system (Hercules, CA, USA) and the ImageJ method. ..

    Article Title: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy
    Article Snippet: .. An ECL chemiluminescence substrate was later added for protein band visualization, whereas ChemiDoc XRS System (Bio-Rad, USA) was applied in imaging. .. Image Lab Software (Bio-Rad) was employed for quantifying band intensities, and GAPDH was an endogenous reference for normalization.

    Article Title: Oroxin A alleviates early brain injury after subarachnoid hemorrhage by regulating ferroptosis and neuroinflammation
    Article Snippet: .. The incubation occurred at ambient temperature for 1.5 h. The protein band visualization and quantification were carried out utilizing a Bio-Rad imaging system (Hercules, CA, USA) and the ImageJ method. ..

    Membrane:

    Article Title: Hyperglycemia promotes tumor immune evasion via B7-H4 upregulation in ovarian cancer.
    Article Snippet: Background: The efficacy of ICB therapy has long been shown to be less prominent in patients with metabolic disorders, including type II diabetes, but the underlying mechanisms remain unclear.. Objective: To investigate how hyperglycemia influences tumor immune evasion and suppresses CD8 T cell function in ovarian cancer, focusing on the role of B7-H4 and AP-1.. Methods: The BKS db/db mice, a model of type II diabetes, and ID8 ovarian cancer cells were used to evaluate tumor growth, immune cell infiltration, and the impact of hyperglycemia on immune checkpoint expression.

    Article Title: Label-free nonlinear optical signatures of extracellular vesicles in liquid and tissue biopsies of human breast cancer
    Article Snippet: .. Resolved proteins were transferred onto 0.22 μm pore-size nitrocellulose membrane, which was subdivided into distinct molecular weight zones, blocked with 3% BSA solution for 2 h and individually immunoblotted with a 1:1000 dilution of either unconjugated Anti-ALIX rabbit monoclonal antibody (Boster-Bio, #M01751), anti-TSG101 rabbit polyclonal antibody (Abcam, #ab30871), anti-CD9 rabbit monoclonal antibody (Boster-Bio, #M01202), anti-CD81 rabbit monoclonal antibody (Boster-Bio, #M01281-1), Anti-GM130/GOLGA2 (Golgi organelle contamination indicator) mouse monoclonal 6D4 antibody (Boster-Bio, # M05865-2) or anti-Calreticulin/CALR (ER organelle presence indicator) (Boster-Bio, #A00894-1) antibody overnight followed by four 7 min wash cycles in prechilled 1X TBS-T buffer, probing with 1:10,000 horse anti-mouse IgG Cell Signaling Technology, #7076S) or 1:40,000 goat anti-rabbit IgG (H + L) (Thermo Fisher, #31460) HRP-linked secondary antibody for 1 h at room temperature, four 5 min washes in 1X TBS-T buffer and a final wash in dH2O prior to chemiluminescent signal development by a 5 min-long blot incubation with SuperSignalTM West Pico PLUS chemiluminescent substrate (Thermo Scientific) and automated protein band visualization in Bio-Rad ChemiDoc MP imaging system at optimal non-saturating 24 s (ALIX), 44 s (TSG101), 180 s (CD9, CD81, GM130 and CALR) exposure time. ..

    Incubation:

    Article Title: Hyperglycemia promotes tumor immune evasion via B7-H4 upregulation in ovarian cancer.
    Article Snippet: Background: The efficacy of ICB therapy has long been shown to be less prominent in patients with metabolic disorders, including type II diabetes, but the underlying mechanisms remain unclear.. Objective: To investigate how hyperglycemia influences tumor immune evasion and suppresses CD8 T cell function in ovarian cancer, focusing on the role of B7-H4 and AP-1.. Methods: The BKS db/db mice, a model of type II diabetes, and ID8 ovarian cancer cells were used to evaluate tumor growth, immune cell infiltration, and the impact of hyperglycemia on immune checkpoint expression.

    Article Title: The mechanism of action in Mussaenda pubescens (Yuye Jinhua) against influenza A virus: Evidence from in vitro and in vivo studies.
    Article Snippet: Background: Influenza A virus (IAV) is recognized as a predominant etiological agent responsible for severe contagious diseases in both humans and animals, characterized by elevated pathogenicity and mortality rates.. Mussaenda pubescens (Yuye Jinhua, YYJH), a traditional medicinal herb known for its anti-inflammatory and detoxifying properties, has been predominantly utilized in conjunction with other therapeutic modalities to manage influenza virus infections.. However, the standalone therapeutic efficacy and underlying mechanisms of YYJH against IAV infection remain insufficiently clarified.

    Article Title: Label-free nonlinear optical signatures of extracellular vesicles in liquid and tissue biopsies of human breast cancer
    Article Snippet: .. Resolved proteins were transferred onto 0.22 μm pore-size nitrocellulose membrane, which was subdivided into distinct molecular weight zones, blocked with 3% BSA solution for 2 h and individually immunoblotted with a 1:1000 dilution of either unconjugated Anti-ALIX rabbit monoclonal antibody (Boster-Bio, #M01751), anti-TSG101 rabbit polyclonal antibody (Abcam, #ab30871), anti-CD9 rabbit monoclonal antibody (Boster-Bio, #M01202), anti-CD81 rabbit monoclonal antibody (Boster-Bio, #M01281-1), Anti-GM130/GOLGA2 (Golgi organelle contamination indicator) mouse monoclonal 6D4 antibody (Boster-Bio, # M05865-2) or anti-Calreticulin/CALR (ER organelle presence indicator) (Boster-Bio, #A00894-1) antibody overnight followed by four 7 min wash cycles in prechilled 1X TBS-T buffer, probing with 1:10,000 horse anti-mouse IgG Cell Signaling Technology, #7076S) or 1:40,000 goat anti-rabbit IgG (H + L) (Thermo Fisher, #31460) HRP-linked secondary antibody for 1 h at room temperature, four 5 min washes in 1X TBS-T buffer and a final wash in dH2O prior to chemiluminescent signal development by a 5 min-long blot incubation with SuperSignalTM West Pico PLUS chemiluminescent substrate (Thermo Scientific) and automated protein band visualization in Bio-Rad ChemiDoc MP imaging system at optimal non-saturating 24 s (ALIX), 44 s (TSG101), 180 s (CD9, CD81, GM130 and CALR) exposure time. ..

    Article Title: Oroxin A alleviates early brain injury after subarachnoid hemorrhage by regulating ferroptosis and neuroinflammation.
    Article Snippet: .. The incubation occurred at ambient temperature for 1.5 h. The protein band visualization and quantification were carried out utilizing a Bio-Rad imaging system (Hercules, CA, USA) and the ImageJ method. ..

    Article Title: Oroxin A alleviates early brain injury after subarachnoid hemorrhage by regulating ferroptosis and neuroinflammation
    Article Snippet: .. The incubation occurred at ambient temperature for 1.5 h. The protein band visualization and quantification were carried out utilizing a Bio-Rad imaging system (Hercules, CA, USA) and the ImageJ method. ..

    Molecular Weight:

    Article Title: Label-free nonlinear optical signatures of extracellular vesicles in liquid and tissue biopsies of human breast cancer
    Article Snippet: .. Resolved proteins were transferred onto 0.22 μm pore-size nitrocellulose membrane, which was subdivided into distinct molecular weight zones, blocked with 3% BSA solution for 2 h and individually immunoblotted with a 1:1000 dilution of either unconjugated Anti-ALIX rabbit monoclonal antibody (Boster-Bio, #M01751), anti-TSG101 rabbit polyclonal antibody (Abcam, #ab30871), anti-CD9 rabbit monoclonal antibody (Boster-Bio, #M01202), anti-CD81 rabbit monoclonal antibody (Boster-Bio, #M01281-1), Anti-GM130/GOLGA2 (Golgi organelle contamination indicator) mouse monoclonal 6D4 antibody (Boster-Bio, # M05865-2) or anti-Calreticulin/CALR (ER organelle presence indicator) (Boster-Bio, #A00894-1) antibody overnight followed by four 7 min wash cycles in prechilled 1X TBS-T buffer, probing with 1:10,000 horse anti-mouse IgG Cell Signaling Technology, #7076S) or 1:40,000 goat anti-rabbit IgG (H + L) (Thermo Fisher, #31460) HRP-linked secondary antibody for 1 h at room temperature, four 5 min washes in 1X TBS-T buffer and a final wash in dH2O prior to chemiluminescent signal development by a 5 min-long blot incubation with SuperSignalTM West Pico PLUS chemiluminescent substrate (Thermo Scientific) and automated protein band visualization in Bio-Rad ChemiDoc MP imaging system at optimal non-saturating 24 s (ALIX), 44 s (TSG101), 180 s (CD9, CD81, GM130 and CALR) exposure time. ..



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